Recovery, Purification, and Cloning of High-Molecular-Weight DNA from Soil Microorganisms

Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms.

We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic librar...

Soil Microorganisms of High-Molecular-Weight DNA from Recovery, Purification, and Cloning

Molecular characterization and DNA methylation profile of Libyodrilus violaceous from oil polluted soil

Studies on earthworms using molecular markers are rare in Africa except a handful from South Africa. Reports on Libyodrilus violaceous,an earthworm found in West Africaare available including their metal tolerance and bioaccumulation capacity but their molecular characterization and ecotoxicology studies are scarce. In this study, triplicate L. violaceous specimens were collec...

Isolation, cloning and gel blot analysis of high molecular weight wheat DNA.

High molecular weight (HMW) DNA analysis is required for physical mapping, contiguous map construction and generating large insert libraries in cosmid, yeast artificial chromosome (YAC) or bacterial artificial chromosome (BAC) vectors (6,7). Procedures of HMW DNA isolation and gel-blot analysis using multi-copy probes have been described for wheat (5). Most of the current HMW DNA isolation proc...

Purification, characterization and molecular cloning of TGP1, a novel G-DNA binding protein from Tetrahymena thermophila.

G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. TGP1 was purified by three-column chromatographies, including a G-DNA affinity ...